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Chromatography of Simulated Plant Pigments

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❶Pour about 2 mL of water into the test tube.

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Furthermore, using too less could lead to difficulty in observation. There was also a risk of cross contamination in the capillary tubes. Advanced Studies Assignment 1. Stability- The product is stable. May cause damage to the following organs: Kidney, liver, mucous membranes, upper respiratory tract, skin, eyes, circulatory system, teeth.

Hazardous in case of eye contact corrosive , of inhalation lung corrosive. Wear appropriate gloves to prevent skin exposure. A respiratory protection program that meets regulations.

Stability- Stable under normal pressure and temperature. Toxicology- Hazardous in case of skin contact irritant, permeator of ingestion, of inhalation, slightly hazardous in case of skin contact sensitizer Silver Nitrate Synonyms- none Chemical Formula- AgNO3 Product is stable under normal conditions.

This substance is toxic to lungs mucous membranes. Slightly hazardous in case of skin contact, ingestion, inhalation. Wear full face shield, full suit, gloves and boots. Avoid reducing agents, moisture, excessive heat and combustible materials. Hazardous in case of skin contact, ingestion, inhalation.

Wear goggles, respirator and gloves. Hazardous in case of skin contact, ingestion and inhalation. Wear goggles, lab coat, respirator and gloves. Ferric Nitrate Nonahydrate Synonyms- none Chemical Formula- Fe NO3 3 Avoid strong oxidizing agents, reducing agents, light, combustible materials and high temperatures. Extremely hazardous in case of skin contact, hazardous in case of ingesting, very hazardous in case of inhalation wear goggles, lab coat, respirator and gloves.

Mercuric Nitrate Monohydrate Synonyms: Hg NO3 2 Avoid high temperatures, light, reducing agents, cyanides and nitrogen oxides. Extremely hazardous in case of ingestion, skin contact and inhalation. K2 CrO4 Avoid heat, light and acids. KI Avoid light, moisture and long exposure to air. Reactive with oxidizing agents, organic materials, metals and acids. Slightly hazardous in case of skin contact ingestion and inhalation.

Wear goggles, lab coat and gloves. To use this method, one must know the possible cations of the mixture. The calculations of this lab give us the calculated Rf value for each cation used in the mixture. This calculation shows the distance the cation moved in relation to the distance the solvent moved. By using determined precise Rf values, a specific cation can be identified.

Rf values are greater when the cation moves a greater distance, and visa versa. The purpose of this lab was fulfilled because each mixture was separated and Rf values were determined. Due to different substances having different solubilities, as the solvent moves across the paper by capillary action, the components begin to separate into diffuse zones.

After the solvent rises up the paper, and the paper is dried and then sprayed with a staining reagent. The staining reagent reacts with the ions, and reveals a different color for each ion. Discussion of Theory cont. In all chromatographic separations, there is an important relationship between the solvent, the chromatography paper, and the mixture.

For a particular mixture, the solvent and the paper must be chosen so the solubility is reversible and be selective for the components of the mixture. The main requirement, though, of the solvent is to dissolve the mixture needing to be separated.

For the separation of a mixture, the substances making up the mixture must be evenly dispersed in a solution, a vapor, or a gas. Once all of the above criteria have been met, chromatography can be a simple tool for separating and comparing chemical mixtures.

Materials The materials used for this lab are paper, pencil, eraser, filter paper, test tube, rubber stopper, paper clip, metric ruler, black felt-tip pen, and a computer. Methods The first step of the method is to bend a paper clip so that it is straight with a hook at one end.

Push the straight end of the paper clip into the bottom of the rubber stopper. Next, you hang a thin strip of filter paper on the hooked end of the paper clip. Insert the paper strip into the test tube. The paper should not touch the sides of the test tube and should almost touch the bottom of the test tube.

Now you will remove the paper strip from the test tube. Draw a solid 5-mm-wide band about 25 mm from the bottom of the paper, using the black felt-tip pen. Use a pencil to draw a line across the paper strip 10 cm above the black band.

Pour about 2 mL of water into the test tube. The water will act as a solvent. Put the filter paper back into the test tube with the bottom of the paper in the water and the black band above the water.

Observe what happens as the liquid travels up the paper. Record the changes you see. When the solvent has reached the pencil line, remove the paper from the test tube. Measure how far the solvent traveled before the strip dries. Finally, let the strip dry on the desk. With the metric ruler, measure the distance from the starting point to the top edge of each color. Record this data in a data table. Calculate a ratio for each color by dividing the distance the color traveled by the distance the solvent traveled.

How many colors separated from the black ink? Five colors separated from the black ink: What served as the solvent for the ink? Water served as the solvent for the ink. As the solvent traveled up the paper, which color of ink appeared first?

The color orange first appeared as the solvent traveled up the paper.


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Paper Chromatography Introduction The purpose of this experiment is to observe how chromatography can be used to separate mixtures of chemical substances. Chromatography serves mainly as a tool for the examination and separation of mixtures of chemical substances.

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The major types are the paper chromatography, thin layer, gas chromatography, column chromatography, High performance liquid chromatography, paper chromatography, thin layer chromatography. There are several applications of paper chromatography and other main types of chromatography techniques.

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In this lab, samples of 5 different cations are placed on chromatography paper, along with a known mixture, which is a mixture of the 5 cations, and 4 unknown substances. The paper was then placed in eluting solution, which is a mixture of HCL, ethanol, and butanol, and then dried and stained with potassium iodide and potassium ferrocyanide. Conclusion The explanation of why different sorts of dye travel at different rates through a mat of cellulose fibres (paper) when a current of water flows through it is roughly as follows. The molecules of a particular dye in water and in contact with cellulose fibres will "partition themselves between the two phases" in a particular proportion.

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Conclusion of Chromatography! Let's assume that you have a compound Z with you which is impure. You want to know the purity of Z (objective). Now, based on the nature you can either go with GC or HPLC. You develope a chromatographic method to anal. Conclusion After completing this lab, each pigment present was separated and identified based on its solubility to the solvent and the R F value of each pigment 93%(14).